mRNA vaccine yields full protection against malaria in mice
Female Anopheles stephensi mosquito obtaining a blood meal from a human host. This mosquito is a known transmitter of malaria, with a distribution that ranges from Egypt all the way to China. Credit: Jim Gathany/CDC
Scientists from the Walter Reed Army Institute of Research and Naval Medical Research Center partnered with researchers at the University of Pennsylvania and Acuitas Therapeutics to develop a novel vaccine based on mRNA technology that protects against malaria in animal models, publishing their findings in npj Vaccines.
In 2019, there were an estimated 229 million cases of malaria and 409,000 deaths globally, creating an extraordinary cost in terms of human morbidity, mortality, economic burden, and regional social stability. Worldwide, Plasmodium falciparum is the parasite species that causes the vast majority of deaths. Those at highest risk of severe disease include pregnant women, children and malaria naive travelers. Malaria countermeasures development has historically been a priority research area for the Department of Defense as the disease remains a top threat to U.S. military forces deployed to endemic regions.
A safe, effective malaria vaccine has long been an elusive target for scientists. The most advanced malaria vaccine is RTS,S, a first-generation product developed in partnership with WRAIR. RTS,S is based on the circumsporozoite protein of P. falciparum, the most dangerous and widespread species of malaria parasite. While RTS,S is an impactful countermeasure in the fight against malaria, field studies have revealed limited sterile efficacy and duration of protection. The limitations associated with RTS,S and other first-generation malaria vaccines have led scientists to evaluate new platforms and second-generation approaches for malaria vaccines.
“Recent successes with vaccines against COVID-19 highlight the advantages of mRNA-based platforms–notably highly targeted design, flexible and rapid manufacturing and ability to promote strong immune responses in a manner not yet explored,” said Dr. Evelina Angov, a researcher at WRAIR’s Malaria Biologics Branch and senior author on the paper. “Our goal is to translate those advances to a safe, effective vaccine against malaria.”
Like RTS,S, the vaccine relies on P. falciparum’s circumsporozoite protein to elicit an immune response. However, rather than administering a version of the protein directly, this approach uses mRNA–accompanied by a lipid nanoparticle that protects from premature degradation and helps stimulate the immune system– to prompt cells to code for circumsporozoite protein themselves. Those proteins then trigger a protective response against malaria but cannot actually cause infection.
“Our vaccine achieved high levels of protection against malaria infection in mice,” said Katherine Mallory, a WRAIR researcher at the time of the article’s submission and lead author on the paper. “While more work remains before clinical testing, these results are an encouraging sign that an effective, mRNA-based malaria vaccine is achievable.”
Materials provided by the Walter Reed Army Insitute of Research. Content may be edited for clarity, style, and length.