Ancient worm reveals way to destroy toxic cells in Huntington’s disease

Neuron_with_mHtt_inclusion
Image shows single striatal neurons transfected with a disease-associated version of huntingtin, the protein that causes Huntington's disease. Nuclei of untransfected neurons are seen in the background (blue). The neuron in the center (yellow) contains an abnormal intracellular accumulation of huntingtin called an inclusion body (orange). Credit: CC BY 3.0 - Dr. Steven Finkbeiner, Gladstone Institute of Neurological Disease, The Taube-Koret Center for Huntington's Disease Research, and the University of California San Francisco.

A research study by associate Professor Roger Pocock, from the Monash Biomedicine Discovery Institute (BDI), and colleagues from the University of Cambridge led by Professor David Rubinsztein, found that microRNAs are important in controlling protein aggregates, proteins that have amassed due to a malfunction in the process of ‘folding’ that determines their shape.

Insights from their study may provide a novel therapeutic approach for diseases such as Huntington’s and Parkinson’s. Their findings were published in eLife.

MicroRNAs, short strands of genetic material, are tiny but powerful molecules that regulate many different genes simultaneously. The scientists sought to identify particular microRNAs that are important for regulating protein aggregates and homed in on miR-1, which is found in low levels in patients with neurodegenerative diseases such as Parkinson’s disease.

“The sequence of miR-1 is 100 percent conserved; it’s the same sequence in the Caenorhabditis elegans worm as in humans even though they are separated by 600 million years of evolution,” Associate Professor Pocock said.

“We deleted miR-1 in the worm and looked at the effect in a preclinical model of Huntington’s and found that when you don’t have this microRNA there’s more aggregation,” he said. “This suggested miR-1 was important to remove Huntington’s aggregates.”

The researchers then showed that miR-1 helped protect against toxic protein aggregates by controlling the expression of the TBC-7 protein in worms. This protein regulates the process of autophagy, the body’s way of removing and recycling damaged cells and is crucial for clearing toxic proteins from cells.

“When you don’t have miR-1, autophagy doesn’t work correctly and you have aggregation of these Huntington’s proteins in worms,” Associate Professor Pocock said.

Professor Rubinsztein then conducted research which showed that the same microRNA regulates a related pathway to control autophagy in human cells.

“Expressing more miR-1 removes Huntington’s aggregates in human cells,” Associate Professor Pocock said.

“It’s a novel pathway that can control these aggregation-prone proteins. As a potential means of alleviating neurodegenerative disease, it’s up there,” he said.

Additional work by Associate Professor Pocock’s colleagues showed that when human cells are supplied with a molecule called interferon beta the miR-1 pathway is upregulated, revealing a way of manipulating it.

He said the studies demonstrated the fundamental importance of discovery research. “We asked a fundamental biological question to dissect a molecular mechanism that now is shown to be really important for potential therapies.”

The researchers have provisionally patented their findings and are in discussions with pharmaceutical companies about translating the research. They will further test it in preclinical models for Huntington’s and Parkinson’s disease.


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Materials provided by Monash University. Content may be edited for clarity, style, and length.


 

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